[FUN_Mail] FUN_Mail Digest, Vol 67, Issue 18
MARY E. MORRISON
Morrison at lycoming.edu
Fri Jul 23 13:03:13 UTC 2021
Greetings Matt,
I set up a group of 4 Nikon E200 scopes equipped for Phase, DAPI/Hoechst, and Alexa488/GFP at Lycoming College, for Cell Biology, Immunology, and Neuroscience lab work, for around $6000 per imaging station. These scopes are durable--they have metal gears, not plastic. Nikon now has filter sets with coatings that don't burn off when students (inevitably) forget and leave the illumination on for extended periods of time with the shutter open. They also have solid state fluorescence sources that last almost forever. But those cost more than halogen bulb systems, so you have to consider your College's budget process--if a bulb burns out, you can usually order a replacement without waiting a year to go through the formal budget process. But if the solid state exciter goes out, that's likely to require a one-year wait for replacement due to the cost. If you order the E200s with a trinocular tube, you can attach pretty inexpensive digital cameras with wifi--like those from Motic, about $400 each, so students can collect high quality images for lab reports. I can provide more details if you contact me directly. I've been very happy with these scopes, and the oldest 2 are more than 10 years old now.
Best regards,
Mary
Mary E. Morrison, Ph.D.
Assistant Professor of Biology
Co-Coordinator of the Neuroscience Program
Lycoming College
Heim Building Room 107
1 College Place
Williamsport, PA 17701
morrison at lycoming.edu
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FUN_Mail Daily Digest
Today's Topics:
1. Epifluorescent teaching microscopes (Matthew Kittelberger)
2. Re: Epifluorescent teaching microscopes (Tamara Stawicki)
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Message: 1
Date: Thu, 22 Jul 2021 18:29:24 +0000
From: Matthew Kittelberger <mkittelb at gettysburg.edu>
To: "fun_mail at lists.funfaculty.org" <fun_mail at lists.funfaculty.org>
Subject: [FUN_Mail] Epifluorescent teaching microscopes
Message-ID: <CE14475B-E0BF-49DF-BC96-B911CED792DC at gettysburg.edu>
Content-Type: text/plain; charset="utf-8"
Hi everyone-
I?m looking for ideas and reviews of decent, but not super-expensive, epifluorescent microscopes for an undergrad cell biology teaching lab. Something that can be used for live imaging of subcellular structures. Since they?re for a teaching lab, we?ll probably ultimately need up to 4 of these, and the Nikon and Zeiss systems I?m used to using for research purposes are probably too expensive. There are some self-contained systems from companies like Keyence and EVOS (by Fisher) that are potentially interesting, and don?t require a dark room. But I?ve heard from some folks that they are more or less just glorified plate readers, which wouldn?t meet our needs. Does anyone have experience working with such systems, and can give me some feedback on that experience? Or does anyone have other suggestions for decent quality systems that won?t break the bank? Thanks so much, Matt Kittelberger
J. Matthew Kittelberger, PhD
Chair, Dept. of Biology
Associate Professor
Department of Biology and Programs in Neuroscience and in Biochemistry and Molecular Biology Gettysburg College Gettysburg, PA 17325 ph. 717.337.6260 mkittelb at gettysburg.edu
------------------------------
Message: 2
Date: Thu, 22 Jul 2021 15:34:50 -0400
From: Tamara Stawicki <stawickt at lafayette.edu>
To: Faculty for Undergraduate Neuroscience Mailing List
<fun_mail at lists.funfaculty.org>
Subject: Re: [FUN_Mail] Epifluorescent teaching microscopes
Message-ID: <3B610657-2904-40A7-9502-F4109CA99205 at lafayette.edu>
Content-Type: text/plain; charset="utf-8"
I have a couple accuscope EXC-350?s (https://accu-scope.com/accuscope-microscopes/by-series/exc-350/exc-350-trinocular-microscope-with-plan-objectives-integrated-led-fluorescence.html <https://accu-scope.com/accuscope-microscopes/by-series/exc-350/exc-350-trinocular-microscope-with-plan-objectives-integrated-led-fluorescence.html>) that I have been very happy with. I have one with 2 color fluorescence that was a little over $6,000 and one with just one color fluorescence that was $4,000 (pricing was from January 2019 so it might have gone up since then). I got them from Ludesco located in Maryland and they actually brought one out for me to demo before I made the purchase but their website isn?t working so I?m not sure if they are still in business. I use them for zebrafish larvae not cell culture, but for what I?m doing (mostly cell counts and looking for fluorescence) I find them comparable if not better than considerably more expensive scopes I?ve worked with. Along those lines I find for my stained fish the signal is bright enough that I don?t need to use them in the dark.
Tamara Stawicki
Assistant Professor of Neuroscience
Lafayette College
309A Oechsle Hall
(610) 330-5287
> On Jul 22, 2021, at 2:29 PM, Matthew Kittelberger <mkittelb at gettysburg.edu> wrote:
>
> Hi everyone-
>
> I?m looking for ideas and reviews of decent, but not super-expensive,
> epifluorescent microscopes for an undergrad cell biology teaching lab.
> Something that can be used for live imaging of subcellular structures.
> Since they?re for a teaching lab, we?ll probably ultimately need up to
> 4 of these, and the Nikon and Zeiss systems I?m used to using for
> research purposes are probably too expensive. There are some
> self-contained systems from companies like Keyence and EVOS (by
> Fisher) that are potentially interesting, and don?t require a dark
> room. But I?ve heard from some folks that they are more or less just
> glorified plate readers, which wouldn?t meet our needs. Does anyone
> have experience working with such systems, and can give me some
> feedback on that experience? Or does anyone have other suggestions
> for decent quality systems that won?t break the bank? Thanks so much,
> Matt Kittelberger
>
> J. Matthew Kittelberger, PhD
> Chair, Dept. of Biology
> Associate Professor
> Department of Biology and Programs in Neuroscience and in Biochemistry
> and Molecular Biology Gettysburg College Gettysburg, PA 17325 ph.
> 717.337.6260 mkittelb at gettysburg.edu
>
>
>
>
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