[FUN_Mail] FUN_Mail Digest, Vol 67, Issue 18

Suren S. Ambegaokar ssambega at owu.edu
Fri Jul 23 14:21:33 UTC 2021


Hi Matt,

I have 2 of the EVOS scopes - one epi with 4 colors and 1 just white light.
I bought them mainly because of their low cost.  The epifluorescent is okay
- the maximum mag is only 20x, which is limiting, but still useful for
detecting fluorescent v. non-fluorescent cells. Trying to use it for
subcellular labeling with IHC/ICC is not great, but you can get a general
idea.  But, with that said, it is VERY easy to use, and I love using it in
my teaching labs. Having a digital screen without oculars is super useful
when teaching, too, and having it all contained without needing a separate
computer makes it really easy to use AND transport to various rooms or
different parts of the room.  It has its limitations, but I think it’s very
useful in a teaching environment. I can send you some images, if you’d like.

Cheers,
Suren

--
Surendra S. Ambegaokar, Ph.D.
Robbins Professor of Neuroscience
Director, Program in Neuroscience
Associate Professor, Dept. of Botany and Microbiology
Ohio Wesleyan University
339 SCSC, 61 S. Sandusky St.
Delaware, OH 43015
ssambega at owu.edu, (O): 740-368-3915



On Fri, Jul 23, 2021 at 8:01 AM <fun_mail-request at lists.funfaculty.org>
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> FUN_Mail Daily Digest
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> Today's Topics:
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>    1. Epifluorescent teaching microscopes (Matthew Kittelberger)
>    2. Re: Epifluorescent teaching microscopes (Tamara Stawicki)
>
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Thu, 22 Jul 2021 18:29:24 +0000
> From: Matthew Kittelberger <mkittelb at gettysburg.edu>
> To: "fun_mail at lists.funfaculty.org" <fun_mail at lists.funfaculty.org>
> Subject: [FUN_Mail] Epifluorescent teaching microscopes
> Message-ID: <CE14475B-E0BF-49DF-BC96-B911CED792DC at gettysburg.edu>
> Content-Type: text/plain; charset="utf-8"
>
> Hi everyone-
>
> I?m looking for ideas and reviews of decent, but not super-expensive,
> epifluorescent microscopes for an undergrad cell biology teaching lab.
> Something that can be used for live imaging of subcellular structures.
> Since they?re for a teaching lab, we?ll probably ultimately need up to 4 of
> these, and the Nikon and Zeiss systems I?m used to using for research
> purposes are probably too expensive.  There are some self-contained systems
> from companies like Keyence and EVOS (by Fisher) that are potentially
> interesting, and don?t require a dark room.  But I?ve heard from some folks
> that they are more or less just glorified plate readers, which wouldn?t
> meet our needs.  Does anyone have experience working with such systems, and
> can give me some feedback on that experience?  Or does anyone have other
> suggestions for decent quality systems that won?t break the bank?  Thanks
> so much, Matt Kittelberger
>
> J. Matthew Kittelberger, PhD
> Chair, Dept. of Biology
> Associate Professor
> Department of Biology and Programs in Neuroscience and in Biochemistry and
> Molecular Biology
> Gettysburg College
> Gettysburg, PA 17325
> ph. 717.337.6260
> mkittelb at gettysburg.edu
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 22 Jul 2021 15:34:50 -0400
> From: Tamara Stawicki <stawickt at lafayette.edu>
> To: Faculty for Undergraduate Neuroscience Mailing List
>         <fun_mail at lists.funfaculty.org>
> Subject: Re: [FUN_Mail] Epifluorescent teaching microscopes
> Message-ID: <3B610657-2904-40A7-9502-F4109CA99205 at lafayette.edu>
> Content-Type: text/plain; charset="utf-8"
>
> I have a couple accuscope EXC-350?s (
> https://accu-scope.com/accuscope-microscopes/by-series/exc-350/exc-350-trinocular-microscope-with-plan-objectives-integrated-led-fluorescence.html
> <
> https://accu-scope.com/accuscope-microscopes/by-series/exc-350/exc-350-trinocular-microscope-with-plan-objectives-integrated-led-fluorescence.html>)
> that I have been very happy with. I have one with 2 color fluorescence that
> was a little over $6,000 and one with just one color fluorescence that was
> $4,000 (pricing was from January 2019 so it might have gone up since then).
> I got them from Ludesco located in Maryland and they actually brought one
> out for me to demo before I made the purchase but their website isn?t
> working so I?m not sure if they are still in business. I use them for
> zebrafish larvae not cell culture, but for what I?m doing (mostly cell
> counts and looking for fluorescence) I find them comparable if not better
> than considerably more expensive scopes I?ve worked with. Along those lines
> I
>  find for my stained fish the signal is bright enough that I don?t need to
> use them in the dark.
>
> Tamara Stawicki
> Assistant Professor of Neuroscience
> Lafayette College
> 309A Oechsle Hall
> (610) 330-5287
>
>
> > On Jul 22, 2021, at 2:29 PM, Matthew Kittelberger <
> mkittelb at gettysburg.edu> wrote:
> >
> > Hi everyone-
> >
> > I?m looking for ideas and reviews of decent, but not super-expensive,
> epifluorescent microscopes for an undergrad cell biology teaching lab.
> Something that can be used for live imaging of subcellular structures.
> Since they?re for a teaching lab, we?ll probably ultimately need up to 4 of
> these, and the Nikon and Zeiss systems I?m used to using for research
> purposes are probably too expensive.  There are some self-contained systems
> from companies like Keyence and EVOS (by Fisher) that are potentially
> interesting, and don?t require a dark room.  But I?ve heard from some folks
> that they are more or less just glorified plate readers, which wouldn?t
> meet our needs.  Does anyone have experience working with such systems, and
> can give me some feedback on that experience?  Or does anyone have other
> suggestions for decent quality systems that won?t break the bank?  Thanks
> so much, Matt Kittelberger
> >
> > J. Matthew Kittelberger, PhD
> > Chair, Dept. of Biology
> > Associate Professor
> > Department of Biology and Programs in Neuroscience and in Biochemistry
> and Molecular Biology
> > Gettysburg College
> > Gettysburg, PA 17325
> > ph. 717.337.6260
> > mkittelb at gettysburg.edu
> >
> >
> >
> >
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-- 
--
Surendra S. Ambegaokar, Ph.D.
Robbins Professor of Neuroscience
Associate Professor, Dept. of Botany and Microbiology
Ohio Wesleyan University
339 SCSC, 61 S. Sandusky St.
Delaware, OH 43015
ssambega at owu.edu, (O): 740-368-3915
*Book an appointment with me on my calendar
<https://calendar.google.com/calendar/selfsched?sstoken=UUtQbWZId1JieVJTfGRlZmF1bHR8YmQzOGE5NWZiYzRlNzUwMDVlNjM0NGRhMWI2ZDQ1M2I>*
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