[FUN_Mail] Rapid Immunohistochemistry?

[Audrey Chen Lew]

Hi Dorothy,

Rory Spence, Mel Coleman and I have used a 3hr protocol successfully in the
classroom with mice brain slices.  The information about the classroom
protocol was published in Biochemistry and Molecular Biology Education:
https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.21611

Happy to answer any questions you may have about it,
Audrey



On Fri, Apr 29, 2022 at 10:25 AM Kozlowski, Dorothy <DKOZLOWS at depaul.edu>
wrote:

> Hi Everyone,
>
> I’m looking for a protocol for a rapid immunohistochemistry for a research
> methods class.  We don’t have the time to do the traditional protocol with
> multiple washes and incubations over two days.  I’ve googled but all I’m
> finding are more clinical protocols for detection of cancer in very thin
> fresh tissue sections.  I have fixed rat brain sections.
>
> Thanks!
>
> Dorothy
>
>
>
> --
>
> Dorothy Kozlowski Ph.D.
>
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-- 
Audrey Chen Lew
Assistant Professor of Teaching
1221 McGaugh Hall
Department of Neurobiology & Behavior
University of California, Irvine
Irvine, CA 92697
(949) 824-5607
Virtual Office: https://uci.zoom.us/my/audreychenlew
<https://www.google.com/url?q=https%3A%2F%2Fuci.zoom.us%2Fmy%2Faudreychenlew>
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